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Image Search Results
Journal: bioRxiv
Article Title: Sex differences in IL-17 determine chronicity in male versus female urinary tract infection
doi: 10.1101/449124
Figure Lengend Snippet: ( A ) Female and male mice were infected with 10 7 CFU of UPEC strain UTI89-RFP-kan R and bladders analyzed by flow cytometry at 24 hours PI. Graphs show ( A ) the number of CD3 + CD4 + T cells, CD3 − NK1.1 + NK cells, γδ T cells, and LTi-like cells (CD90 + CD25 + CD4 + CD3 − NK1.1 − MHC II − CD11b − ) in bladders. ( B ) Female mice were implanted with empty tubing (Mock) or slow release tubing containing testosterone (T tube) and allowed to recover 1 week before infection with 10 7 CFU UPEC strain UTI89-RFP-kan R . Graphs show the number of CD3 + CD4 + T cells, CD3 − NK1.1 + NK cells, γδ T cells, and CD90 + CD25 + CD4 + CD3 − NK1.1 − MHC II − CD11b − LTi-like cells in bladders. ( C-E ) Graphs show the number of infected mice, determined by urine sampling, over time in ( C ) wildtype or RAG2 − /-γc mice, ( D ) mice treated with isotype or αNK1.1-depleting antibody, ( E ) mice treated with α-IL-17 neutralizing antibody. ( A-B ) Data are pooled from 2-3 experiments, n=4-5/group in each experiment. Each dot is one mouse, red dots depict female mice and blue dots are male mice, lines are medians. ( C-E ) Graphs are representative experiments, n=6-12 mice per experiment, at least 2 experiments each. * p<0.05, ** p<0.01, Mann-Whitney test. Analyses in this figure were corrected for multiple testing by Holm–Bonferroni method, all p<0.05 had q< 0.05.
Article Snippet: For IL-17 neutralization, 83.3-111
Techniques: Infection, Flow Cytometry, Sampling, MANN-WHITNEY